Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
Mem. Inst. Oswaldo Cruz
;
104(8): 1055-1062, Dec. 2009. ilus
Article
Dans Anglais
| LILACS
| ID: lil-538164
ABSTRACT
Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Proline
/
Antienzymes
/
Amino-acid isomerases
/
Interactions hôte-parasite
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2009
Type:
Article
Pays d'affiliation:
Brésil
/
France
Institution/Pays d'affiliation:
Institut Pasteur/FR
/
Instituto Oswaldo Cruz-Fiocruz/BR
/
Laboratório de Genômica Funcional e Bioinformática/BR
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