Expression of Hepatitis B virus surface antigen (HBsAg) from genotypes A, D and F and influence of amino acid variations related or not to genotypes on HBsAg detection
Braz. j. infect. dis
;
13(4): 266-271, Aug. 2009. graf, ilus
Article
Dans Anglais
| LILACS
| ID: lil-539761
ABSTRACT
The impact of hepatitis B virus (HBV) genotypes on the sensitivity of surface antigen (HBsAg) detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37 percent and 30 percent lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44 percent and an increase of 34 percent, respectively, on HBsAg mean values in comparison with their consensus forms. In conclusion, HBsAg detection levels varied among HBV genotypes. However, unique amino acid substitutions not linked to genotypes, such as T125M and T143M described here, should have more implications in HBV immunological diagnostics than the set of variations characteristic of each HBV genotype.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
ADN viral
/
Virus de l'hépatite B
/
Hépatite B
/
Antigènes de surface du virus de l'hépatite B
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. infect. dis
Thème du journal:
Maladies transmissibles
Année:
2009
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
FIOCRUZ/BR
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