Efeito do licor de absinto e do seu princípio ativo (thujone) proveniente da losna (arthemisia absinthium) nas glândulas salivares de ratos / Effect of the absinthe and its active component (thujone) of the Arthemisia absinthium on the salivary glands of the rats

RESUMO

Introdução - O objetivo do presente estudo foi avaliar , através do microscópio de luz , as glândulas salivares de ratos tratados com álcool, absinto ou seu princípio ativo, thujone (extraído da Arthemisia absinthium ). Material e Métodos - Vinte ratos machos (Wistar) foram divididos em quatro grupos controle, tratados com álcool, absinto, e thujone. A administração das drogas foi feita por via oral, controlada diariamente através do volume ingerido. Os animais do grupo controle sofreram os mesmos procedimentos com água comum. Após 13 dias do início do experimento, os ratos foram sacrificados, sendo suas glândulas salivares removidas e submetidas às técnicas laboratoriais e de coloração, para a análise em microscópio comum. Resultados - Foram observadas alterações morfológicas nos ratos que receberam álcool, thujone e absinto. As glândulas salivares dos ratos tratados com absinto apresentaram alterações mais acentuadas, ilustradas pela presença de células dismórficas, atrofia e fibrose. Conclusão - Pelos resultados encontrados, o absinto exacerba as lesões celulares induzidas pelo álcool nas glândulas salivares de ratos.

ABSTRACT

Introduction - The aim of this study was to evaluate on light microscopy the glands salivary of rats treated with ethanol, absinthe and its active principle, thujone (removed from Arthemisia absinthium). Material and Methods - Twenty male rats (Wistar) were used that were equally divided in 4 groups control, treated with absinthe, treated with ethanol and, the last, treated with thujone. The drugs administration was done in oral way, daily checked trough the control of ingested volume. The animals of the control group had the same procedure, with common water. After 13 days of the start of the experiment, the rats were killed with anesthetic overdose and their glands salivary were removed a submitted to the laboratorial routine process to the embedding on paraffin. The cuts were stained using H&E and analysed on light microscopy. Results - Morphological changes were observed on the glands salivary of the rats treated with absinthe, thujone and ethanol. The glands salivary of the rats treated with absinthe showed more accentuated alterations, comparing with the animals treated with ethanol or thujone, showed by dimorphism cell, atrophy and fibrosis. Conclusion - The results indicated that absinthe exacerbate the cellular lesions induced by ethanol that occur on the salivary glands in rats.