Toxicity of chlorhexidine on odontoblast-like cells
J. appl. oral sci
; 18(1): 50-58, Jan.-Feb. 2010. tab, ilus
Article
Dans En
| LILACS
| ID: lil-545027
Responsable en Bibliothèque :
BR1.1
ABSTRACT
Chlorhexidine gluconate (CHX) is recommended for a number of clinical procedures and it has been pointed out as a potential cavity cleanser to be applied before adhesive restoration of dental cavities. OBJECTIVE:
As CHX may diffuse through the dentinal tubules to reach a monolayer of odontoblasts that underlies the dentin substrate, this study evaluated the cytotoxic effects of different concentrations of CHX on cultured odontoblast-like cells (MDPC-23). MATERIAL ANDMETHODS:
Cells were cultured and exposed to CHX solutions at concentrations of 0.06 percent, 0.12 percent, 0.2 percent, 1 percent and 2 percent. Pure culture medium (á-MEM) and 3 percent hydrogen peroxide were used as negative and positive control, respectively. After exposing the cultured cells to the controls and CHX solutions for 60 s, 2 h or 60 s with a 24-h recovery period, cell metabolism (MTT assay) and total protein concentration were evaluated. Cell morphology was assessed under scanning electron microscopy. CHX had a dose-dependent toxic effect on the MDPC-23 cells.RESULTS:
Statistically significant difference was observed when the cells were exposed to CHX in all periods (p<0.05). Significant difference was also determined for all CHX concentrations (p<0.05). The 60-s exposure time was the least cytotoxic (p<0.05), while exposure to CHX for 60 s with a 24-h recovery period was the most toxic to the cells (p<0.05).CONCLUSION:
Regardless of the exposure time, all CHX concentrations had a high direct cytotoxic effect to cultured MDPC-23 cells.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Chlorhexidine
/
Anti-infectieux locaux
/
Odontoblastes
Limites du sujet:
Humans
langue:
En
Texte intégral:
J. appl. oral sci
Thème du journal:
ODONTOLOGIA
Année:
2010
Type:
Article
/
Project document