Characterization of 23S rRNA domain V mutations in gastric biopsy patients from the eastern Amazon
Mem. Inst. Oswaldo Cruz
; 105(3): 314-317, May 2010. tab
Article
de En
| LILACS
| ID: lil-547302
Bibliothèque responsable:
BR1.1
ABSTRACT
Resistance of Helicobacter pylori to clarithromycin is characterised by simple point mutations in the 23S ribosomal RNA (rRNA) gene and is responsible for the majority of cases of failure to eradicate this bacterium. In this paper, we characterised the variability of the 23S rRNA gene in biopsies of patients with gastric pathologies in the eastern Amazon (Northern Region of Brazil) using PCR and sequencing. A total of 49 sequences of H. pylori strains were analysed and of those, 75.6 percent presented nucleotide substitutions A2142G (3.3 percent), T2182C (12.9 percent), G2224A (6.45 percent), T2215C (61.3 percent), A2192G (3.3 percent), G2204C (6.4 percent) and T2221C (6.4 percent). Of the mutations identified, four are known mutations related to cases of resistance and 16.1 percent are not yet described, revealing a high prevalence of mutations in the H. pylori 23S rRNA gene among the strains circulating in the in the eastern Amazon. The high prevalence in individuals with gastric pathologies in the Northern Region of Brazil demonstrates the need for characterising the profile of these strains to provide correct therapy for patients, considering that mutations in this gene are normally associated with resistance to the primary medication used in controlling H. pylori infection.
Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Maladies de l'estomac
/
Helicobacter pylori
/
Infections à Helicobacter
/
Mutation ponctuelle
/
Résistance bactérienne aux médicaments
Type d'étude:
Risk_factors_studies
Limites du sujet:
Humans
Pays comme sujet:
America do sul
/
Brasil
langue:
En
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
MEDICINA TROPICAL
/
PARASITOLOGIA
Année:
2010
Type:
Article