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Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
Costa, V. M; Langoni, H.
  • Costa, V. M; São Paulo State University. Veterinary Medicine and Animal Husbandry School. Zoonosis Research Center. Botucatu. BR
  • Langoni, H; São Paulo State University. Veterinary Medicine and Animal Husbandry School. Zoonosis Research Center. Botucatu. BR
J. venom. anim. toxins incl. trop. dis ; 16(2): 368-374, 2010. tab
Article Dans Anglais | LILACS | ID: lil-548856
ABSTRACT
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Toxoplasma / Dosage biologique / Rat Wistar / Lait Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: Toxicologie Année: 2010 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: São Paulo State University/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Toxoplasma / Dosage biologique / Rat Wistar / Lait Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: Toxicologie Année: 2010 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: São Paulo State University/BR