Peptidolytic microbial community of methanogenic reactors from two modified UASBs of brewery industries
Braz. j. microbiol
;
41(3): 707-717, Oct. 2010. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-549412
ABSTRACT
We studied the peptide-degrading anaerobic communities of methanogenic reactors from two mesophilic full-scale modified upflow anaerobic sludge blanket (UASB) reactors treating brewery wastewater in Colombia. Most probable number (MPN) counts varied between 7.1 x 10(8) and 6.6 x 10(9) bacteria/g volatile suspended solids VSS (Methanogenic Reactor 1) and 7.2 x 10(6) and 6.4 x 10(7) bacteria/g (VSS) (Methanogenic Reactor 2). Metabolites detected in the highest positive MPN dilutions in both reactors were mostly acetate, propionate, isovalerate and, in some cases, negligible concentrations of butyrate. Using the highest positive dilutions of MPN counts, 50 dominant strains were isolated from both reactors, and 12 strains were selected for sequencing their 16S rRNA gene based on their phenotypic characteristics. The small-subunit rRNA gene sequences indicated that these strains were affiliated to the families Propionibacteriaceae, Clostridiaceae and Syntrophomonadaceae in the low G + C gram-positive group and Desulfovibrio spp. in the class d-Proteobacteria. The main metabolites detected in the highest positive dilutions of MPN and the presence of Syntrophomonadaceae indicate the effect of the syntrophic associations on the bioconversion of these substrates in methanogenic reactors. Additionally, the potential utilization of external electron acceptors for the complete degradation of amino acids by Clostridium strains confirms the relevance of these acceptors in the transformation of peptides and amino acids in these systems.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Peptides
/
ARN bactérien
/
Séquence nucléotidique
/
Stabilité de l'ARN
/
Réacteurs Biologiques Séquentiels
/
Eaux usées
/
Bactéries anaérobies à Gram négatif
Type d'étude:
Études d'évaluation
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2010
Type:
Article
Pays d'affiliation:
Australie
/
Colombie
/
France
Institution/Pays d'affiliation:
Griffith University/AU
/
Pontificia Universidad Javeriana/CO
/
Universités de Provence et de la Méditerranée/FR
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