Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation
Braz. arch. biol. technol
;
53(4): 883-888, July-Aug. 2010. graf, tab
Article
Dans Anglais
| LILACS
| ID: lil-554783
ABSTRACT
Nodal segments from greenhouse grown adult plants of Salvia guaranitica were used to evaluate the effect of culture media and growth regulators on the micropropagation and growth. The highest multiplication rate was obtained on Murashige and Skoog (MS) medium supplemented with 2.22 µM of 6-benzylaminopurine (BAP). The best condition for rooting was MS medium with 2.85 µM indole-3-acetic acid (IAA). Rooted plants were successfully acclimatized and exhibited a normal development until maturity. Using the described protocol, approximately 35 plants per explant were obtained after three months.
RESUMO
Segmentos nodais de plantas adultas de Salvia guaranitica cultivadas em estufa, foram utilizados para avaliar o efeito do meio de cultivo e reguladores de crescimento na micropropagação. A maior taxa de multiplicação foi obtida em meio Murashige and Skoog (MS) suplementado com 2.22 µM de 6-benzilaminopurina (BAP). A melhor condição para enraizamento foi o meio MS acrescido de 2.85 µM de ácido indol-3-acético (IAA). Plântulas enraizadas foram aclimatizadas com sucesso e exibiram desenvolvimento normal até a fase adulta. Utilizando o protocolo descrito, aproximadamente 35 plantas por explante foram obtidas após 3 meses.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
langue:
Anglais
Texte intégral:
Braz. arch. biol. technol
Thème du journal:
Biologie
Année:
2010
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de Caxias do Sul/BR
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