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Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)
Tavares, Mara Garcia; Carvalho, Carlos Roberto; Soares, Fernanda Aparecida Ferrari; Fernandes, Anderson.
  • Tavares, Mara Garcia; Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa. BR
  • Carvalho, Carlos Roberto; Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa. BR
  • Soares, Fernanda Aparecida Ferrari; Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa. BR
  • Fernandes, Anderson; Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa. BR
Genet. mol. biol ; 33(3): 491-493, 2010. graf, tab
Article Dans Anglais | LILACS | ID: lil-555809
ABSTRACT
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Abeilles / Analyse cytogénétique / Hymenoptera Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: Genet. mol. biol Thème du journal: Génétique Année: 2010 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal de Viçosa/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Abeilles / Analyse cytogénétique / Hymenoptera Type d'étude: Etude diagnostique Limites du sujet: Animaux langue: Anglais Texte intégral: Genet. mol. biol Thème du journal: Génétique Année: 2010 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal de Viçosa/BR