Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
Mem. Inst. Oswaldo Cruz
;
105(5): 706-711, Aug. 2010. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-557234
ABSTRACT
The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Fibrinogène
/
Corynebacterium diphtheriae
/
Toxine diphtérique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2010
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Fiocruz/BR
/
Universidade do Estado do Rio de Janeiro/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS