Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
J. venom. anim. toxins incl. trop. dis
; J. venom. anim. toxins incl. trop. dis;16(4): 537-542, 2010.
Article
de En
| LILACS
| ID: lil-566169
Bibliothèque responsable:
BR33.1
ABSTRACT
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.
Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Réaction en chaîne par ligase
/
Leishmaniose viscérale
Type d'étude:
Diagnostic_studies
langue:
En
Texte intégral:
J. venom. anim. toxins incl. trop. dis
/
J. venom. anim. toxins incl. trop. dis. (CD-ROM)
/
Journal of venomous animals and toxins including tropical diseases (Online)
Thème du journal:
TOXICOLOGIA
Année:
2010
Type:
Article