Increased levels of glutamate in the central nervous system are associated with behavioral symptoms in experimental malaria
Braz. j. med. biol. res
;
43(12): 1173-1177, Dec. 2010. ilus
Article
Dans Anglais
| LILACS
| ID: lil-569001
ABSTRACT
Cerebral malaria (CM) is a severe complication resulting from Plasmodium falciparum infection. This condition has been associated with cognitive, behavioral and motor dysfunctions, seizures and coma. The underlying mechanisms of CM are incompletely understood. Glutamate and other metabolites such as lactate have been implicated in its pathogenesis. In the present study, we investigated the involvement of glutamate in the behavioral symptoms of CM. Seventeen female C57BL/6 mice (20-25 g) aged 6-8 weeks were infected with P. berghei ANKA by the intraperitoneal route using a standardized inoculation of 10(6) parasitized red blood cells suspended in 0.2 mL PBS. Control animals (N = 17) received the same volume of PBS. Behavioral and neurological symptoms were analyzed by the SmithKline/Harwell/Imperial College/Royal Hospital/Phenotype Assessment (SHIRPA) battery. Glutamate release was measured in the cerebral cortex and cerebrospinal fluid of infected and control mice by fluorimetric assay. All functional categories of the SHIRPA battery were significantly altered in the infected mice at 6 days post-infection (dpi) (P ≤ 0.05). In parallel to CM symptoms, we found a significant increase in glutamate levels in the cerebral cortex (mean ± SEM; control 11.62 ± 0.90 nmol/mg protein; infected at 3 dpi 10.36 ± 1.17 nmol/mg protein; infected at 6 dpi 26.65 ± 0.73 nmol/mg protein; with EGTA, control 5.60 ± 1.92 nmol/mg protein; infected at 3 dpi 6.24 ± 1.87 nmol/mg protein; infected at 6 dpi 14.14 ± 0.84 nmol/mg protein) and in the cerebrospinal fluid (control 128 ± 51.23 pmol/mg protein; infected 301.4 ± 22.52 pmol/mg protein) of infected mice (P ≤ 0.05). These findings suggest a role of glutamate in the central nervous system dysfunction found in CM.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Plasmodium berghei
/
Symptômes comportementaux
/
Cortex cérébral
/
Liquide cérébrospinal
/
Paludisme cérébral
/
Acide glutamique
Type d'étude:
Etude diagnostique
/
Facteurs de risque
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2010
Type:
Article
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