Nova técnica para contagem do número de células de blastocistos / A new technique to count the number of cells of blastocysts
Arq. bras. med. vet. zootec
;
62(6): 1507-1510, dez. 2010. ilus
Article
Dans Portugais
| LILACS
| ID: lil-576055
ABSTRACT
A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this new method shows advantages related to the cell visualization, which can be done in more simple equipment like stereoscopic microscope. Other interesting observed point was the long period of time and quality that the coloration stays on slides, considering other techniques.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Ovis
/
Muridae
Limites du sujet:
Animaux
langue:
Portugais
Texte intégral:
Arq. bras. med. vet. zootec
Thème du journal:
Médecine vétérinaire
Année:
2010
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
GERMOVET/BR
/
Universidade Federal de Viçosa/BR
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