Human sulfatase transiently and functionally active expressed in E. coli K12
Electron. j. biotechnol
;
13(3): 5-6, May 2010. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-577100
ABSTRACT
The recombinant human iduronate 2-sulfate sulfatase (hrIDS) was transiently and functionally active expressed in E. coli K12. The enzyme activity (crude extract) at 100 ml and 400 ml oscillated between 0.25 and 10.58 nmol h-1 mg-1. The wide Western-blot peptide profile suggest that hrIDS is proteolitically processed randomly which agrees with the ultrafiltration assay in which the hrIDS activity was found in all fractions (<30kDa, 30-100kDa and >100kDa). No glycation sites were found by computer analysis of the hIDS sequence; discarding the possibility of marks for glycation and proteolytic processing.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines recombinantes
/
Iduronate 2-sulfatase
langue:
Anglais
Texte intégral:
Electron. j. biotechnol
Thème du journal:
Biotechnologie
Année:
2010
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Colombie
Institution/Pays d'affiliation:
Pontificia Universidad Javeriana/CO
/
Universidad del Quindío/CO
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