Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn)
Biol. Res
;
43(4): 385-392, 2010. ilus
Article
Dans Anglais
| LILACS
| ID: lil-582852
ABSTRACT
Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10 percent fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10 percent FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72 percent, respectively, after six months of storage in liquid nitrogen (-196 ° C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Carpes (poisson)
/
Lignée cellulaire
/
Techniques de culture cellulaire
/
Nageoires animales
/
Myocarde
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2010
Type:
Article
Pays d'affiliation:
Inde
Institution/Pays d'affiliation:
National Bureau of Fish Genetic Resources/IN
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS