Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura

Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura.

Miyata, Marcelo; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara. BR
Santos, Adolfo Carlos Barreto; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara. BR
Mendes, Natália Helena; s.af
Cunha, Eunice Atsuko; s.af
Melo, Fernando Augusto Fiúza de; s.af
Leite, Clarice Queico Fujimura; s.af

Braz. j. microbiol ; 42(2): 774-777, Apr.-June 2011. ilus

Article Dans Anglais | LILACS | ID: lil-590022

ABSTRACT

ABSTRACT

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

CTAB; DNA extraction; Mycobacterium tuberculosis; Thermolysis

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Texte intégral: Disponible Indice: LILAS (Amériques) Type d'étude: Etude diagnostique langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2011 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual Paulista/BR

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