Antiherpes screening of marine organisms from Colombian Caribbean Sea
Rev. bras. farmacogn
;
21(4): 608-614, jul.-ago. 2011. tab
Article
Dans Anglais
| LILACS
| ID: lil-596246
ABSTRACT
The exploration of marine environment represents a promising strategy in the search for new active antiviral compounds. The isolation and characterization of the nucleosides spongothymidine and spongouridine from the sponge Cryptotethia crypta used as models for the synthesis of ara-A (vidarabine), that has been used therapeutically against herpetic encephalitis, was the most important contribution since the late 1970s. This paper describes the in vitro antiviral evaluation of 26 organic extracts obtained from eleven octocoral species and fifteen marine sponges. Cytotoxicity was evaluated on Vero cells by MTT assay and the antiviral activity was tested against Herpes Simplex Virus type 1 (HSV-1, KOS strain) by plaque number reduction assay. Results were expressed as 50 percent cytotoxic (CC50) and 50 percent inhibitory (IC50) concentrations, respectively, in order to calculate the selectivity index (SI= CC50/IC50) of each extract. Among the tested marine octocoral species, only three extracts showed antiviral activity, but with low selectivity indices (<3.0). Among the tested marine sponges, eight extracts showed SI values higher than 2.0, and three can be considered promising (Aka cachacrouense, Niphates erecta and Dragmacidon reticulatum) with SI values of 5.0, 8.0 and 11.7, respectively, meriting complementary studies in order to identify the bioactive components of these sponge extracts, which are in course now.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Pays comme sujet:
Amérique du Sud
/
Colombie
langue:
Anglais
Texte intégral:
Rev. bras. farmacogn
Thème du journal:
Pharmacie
Année:
2011
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
/
Colombie
Institution/Pays d'affiliation:
Universidad Nacional de Colombia/CO
/
Universidade Federal de Santa Catarina/BR
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