Aumento del daño en el ADN espermático en varones mayores de 40 años / Association of age with sperm DNA fragmentation
Rev. méd. Chile
;
139(3): 306-312, mar. 2011. ilus
Article
Dans Espagnol
| LILACS
| ID: lil-597618
ABSTRACT
Background:
There is an association between aging ana an increased number ofsperms with alterations in nuclear DNA.Aim:
To study the association between age and fragmentation of sperm DNA. Material andMethods:
Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TÚNEL (terminal deoxynucleotidyl transferase-mediated2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersión test) assays.Results:
Compared with theiryounger counterparts, patients aged more than 40years had a higher proportion ofsperms with DNA fragmentation by TÚNEL (20 ±1.3 and24 ± 1.9 percent respectively, p < 0.05) and SCD (22 ± 1.4 and26 ± 1.6 percent respectively, p < 0.05). The results ofboth assays had a correlation coefficientofO.8. No differences between groups were observed for other seminal parameters.Conclusions:
Sperm DNA fragmentation increases with age in males.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Spermatozoïdes
/
Chromatine
/
Méthode TUNEL
/
Fragmentation de l'ADN
/
Analyse du sperme
Type d'étude:
Facteurs de risque
Limites du sujet:
Adolescent
/
Adulte
/
Humains
/
Mâle
langue:
Espagnol
Texte intégral:
Rev. méd. Chile
Thème du journal:
Médicament
Année:
2011
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chili
Institution/Pays d'affiliation:
Universidad de Chile/CL
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