Kinetic properties of poly (2'-0-methycytidylic acid)- directed reverse transcriptase reaction
Braz. j. med. biol. res
;
21(3): 445-8, Mar. 1988. tab
Article
Dans Anglais
| LILACS
| ID: lil-60214
RESUMO
Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide
Recherche sur Google
Indice:
LILAS (Amériques)
Sujet Principal:
Poly C
/
RNA-directed DNA polymerase
/
Guanosine triphosphate
/
Virus de la myéloblastose aviaire
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1988
Type:
Article
/
descriptif de projet
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS