Comparação do isolamento microbiológico e da reação em cadeia da polimerase no diagnóstico de salmonelose em bezerros infectados experimentalmente com Salmonella Typhimurium / Comparison of microbiological culture and polymerase chain reaction in the diagnosis of salmonellosis in Salmonella Typhimurium-infected calves
Arq. bras. med. vet. zootec
;
63(5): 1241-1245, out. 2011. ilus, tab
Article
Dans Portugais
| LILACS
| ID: lil-605854
ABSTRACT
The efficiency of microbiological culture and polymerase chain reaction (PCR) for detection of Salmonella Typhimurium is compared in fecal samples of Holstein calves experimentally infected with 10(9) CFU of Salmonella Typhimurium. Seventy-two fecal samples were analyzed by microbiological culture and PCR associated with selenite cystine (SC) and Muller-Kauffmann tethrationate (TMK) selective enrichment broths. Regardless of the selective enrichment broth, the microbiological culture was significantly better than PCR for detection of positive samples of Salmonella Typhimurium. The selective enrichment broths SC and TMK had no effect on the efficiency of the microbiological culture. The SC broth was the best option as selective enrichment associated to PCR.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Bactéries
/
Bovins
/
Réaction de polymérisation en chaîne
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Portugais
Texte intégral:
Arq. bras. med. vet. zootec
Thème du journal:
Médecine vétérinaire
Année:
2011
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
UNESP/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS