Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica
Braz. j. microbiol
;
42(3): 868-877, July-Sept. 2011. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-607515
ABSTRACT
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100 percent identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Streptomyces
/
Beta-Amylase
/
ARN bactérien
/
Microbiologie de l'environnement
/
Amylases
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2011
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Danemark
/
Roumanie
Institution/Pays d'affiliation:
Romanian Polar Research Institute/RO
/
University Dunãrea de Jos/RO
/
University of Copenhagen/DK
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