Histomorphometric assessment of bone necrosis produced by two cryosurgery protocols using liquid nitrogen: an experimental study on rat femurs
J. appl. oral sci
;
19(6): 604-609, Nov.-Dec. 2011. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-610874
ABSTRACT
OBJECTIVE:
The aim of this study was to evaluate the effects of liquid nitrogen cryosurgery on the femoral diaphysis of rats. MATERIAL ANDMETHODS:
The femoral diaphyses of 42 Wistar rats were exposed to three local and sequential applications of liquid nitrogen for 1 or 2 min, intercalated with periods of 5 min of passive thawing. The animals were sacrificed after 1, 2, 4 and 12 weeks and the specimens obtained were processed and analyzed histomorphometrically.RESULTS:
The depth and extent of peak bone necrosis were 124.509 µm and 2087.094 µm for the 1-min protocol, respectively, and 436.424 µm and 12046.426 µm for the 2-min protocol. Peak necrosis was observed in the second experimental week with both cryotherapy protocols.CONCLUSIONS:
The present results indicate that the 2-min protocol produced more marked bone necrosis than the 1-min protocol. Although our results cannot be entirely extrapolated to clinical practice, they contribute to the understanding of the behavior of bone tissue submitted to different cycles of liquid nitrogen freezing and may serve as a basis for new studies.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cryochirurgie
/
Fémur
/
Nécrose de la tête fémorale
/
Azote
Type d'étude:
Études d'évaluation
/
Guide de pratique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
J. appl. oral sci
Thème du journal:
Dentisterie
Année:
2011
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Federal University of Ceará/BR
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