Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Braz. j. infect. dis
;
15(5): 467-472, Sept.-Oct. 2011. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-612706
ABSTRACT
BACKGROUND:
It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner.OBJECTIVES:
Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene.METHODS:
Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay.RESULTS:
The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV, 37 (28.9 percent) were positive for the virus by both methods and 25 samples were positive only by nested-PCR (67.5 percent increase in detection rate compared with single PCR). All HPV positive samples were effectively typed by RFLP assay.CONCLUSION:
The method of nested-PCR proved to be an effective diagnostic tool for HPV detection and typing.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Papillomaviridae
/
Col de l'utérus
/
Infections à papillomavirus
Type d'étude:
Etude diagnostique
Limites du sujet:
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Braz. j. infect. dis
Thème du journal:
Maladies transmissibles
Année:
2011
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Simbios Biotecnologia/BR
/
Universidade Luterana do Brasil/BR
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