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Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Coser, Janaina; Boeira, Thaís da Rocha; Fonseca, André Salvador Kazantzi; Ikuta, Nilo; Lunge, Vagner Ricardo.
  • Coser, Janaina; Universidade Luterana do Brasil. Postgraduate Program in Genetics and Molecular Diagnosis. Canoas. BR
  • Boeira, Thaís da Rocha; Simbios Biotecnologia. Canoas. BR
  • Fonseca, André Salvador Kazantzi; Simbios Biotecnologia. Canoas. BR
  • Ikuta, Nilo; Universidade Luterana do Brasil. Postgraduate Program in Genetics and Molecular Diagnosis. Canoas. BR
  • Lunge, Vagner Ricardo; Universidade Luterana do Brasil. Postgraduate Program in Genetics and Molecular Diagnosis. Canoas. BR
Braz. j. infect. dis ; 15(5): 467-472, Sept.-Oct. 2011. ilus, tab
Article Dans Anglais | LILACS | ID: lil-612706
ABSTRACT

BACKGROUND:

It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner.

OBJECTIVES:

Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene.

METHODS:

Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay.

RESULTS:

The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV, 37 (28.9 percent) were positive for the virus by both methods and 25 samples were positive only by nested-PCR (67.5 percent increase in detection rate compared with single PCR). All HPV positive samples were effectively typed by RFLP assay.

CONCLUSION:

The method of nested-PCR proved to be an effective diagnostic tool for HPV detection and typing.
Sujets)


Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Papillomaviridae / Col de l'utérus / Infections à papillomavirus Type d'étude: Etude diagnostique Limites du sujet: Femelle / Humains langue: Anglais Texte intégral: Braz. j. infect. dis Thème du journal: Maladies transmissibles Année: 2011 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Simbios Biotecnologia/BR / Universidade Luterana do Brasil/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Papillomaviridae / Col de l'utérus / Infections à papillomavirus Type d'étude: Etude diagnostique Limites du sujet: Femelle / Humains langue: Anglais Texte intégral: Braz. j. infect. dis Thème du journal: Maladies transmissibles Année: 2011 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Simbios Biotecnologia/BR / Universidade Luterana do Brasil/BR