ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine
Braz. j. microbiol
;
42(4): 1420-1426, Oct.-Dec. 2011. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-614604
ABSTRACT
Haemophilus parasuis infection, known as Glãsser's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 percent), followed by serovars 14 (14 percent), 5 (12 percent), 13 (8 percent) and 2 (2 percent), whereas 40 percent of the strains were considered as non-typeable. From 50 strains tested 43 (86 percent) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Tests sérologiques
/
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Haemophilus parasuis
/
Infections à Haemophilus
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2011
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de São Paulo/BR
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