Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Braz. j. microbiol
;
42(4): 1440-1444, Oct.-Dec. 2011. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-614608
ABSTRACT
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Infections à respirovirus
/
Phylogenèse
/
Techniques in vitro
/
Séquence nucléotidique
/
RT-PCR
/
Transcription inverse
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2011
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal do Rio Grande do Sul/BR
/
Universidade Feevale/BR
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