A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
Braz. arch. biol. technol
;
55(1): 1-6, Jan.-Feb. 2012. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-622675
ABSTRACT
Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
langue:
Anglais
Texte intégral:
Braz. arch. biol. technol
Thème du journal:
Biologie
Année:
2012
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Católica de Santos/BR
/
Universidade de Caxias do Sul/BR
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