The cell biology of mosquito vitellogenesis
Mem. Inst. Oswaldo Cruz
;
82(supl.3): 93-101, 1987. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-623745
ABSTRACT
Insect vitellogenesis involves coordinated activities of the fat body and oocytes. We have studied these activities at the cellular level in the mosquito. During each vitellogenic cycle, the fat body undergoes three successive stages 1) proliferation of biosynthetic organelles, 2) vitellogenin synthesis, 3) termination of vitellogenin synthesis and degradation of biosynthetic organelles by lysosomes. Analysis with monoclonal antibodies and radiolabelling demonstrated that the mosquito yolk protein consists of two subunits (200-kDa and 65-kDa). Both subunits are glycosylated, their carbohydrate moieties are composed of high-mannose oligosaccharides. The yolk protein subunits are derived from a single 220 kDa precursor detected by an in vitro translation. Oocytes become competent to internalize proteins as a result of juvenile hormone-mediated biogenesis of endocytotic organelles. The yolk protein is then accumulated by receptor-mediated endocytosis. A pathway of the yold protein and factors determining its routing in the oocyte have been studied.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Vitellogenèse
/
Lutte contre les moustiques
/
Biologie cellulaire
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1987
Type:
Article
/
Congrès et conférence
/
descriptif de projet
Pays d'affiliation:
États-Unis d'Amérique
Institution/Pays d'affiliation:
Michigan State University/US
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