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ANALYSIS OF TWO POPULATIONS OF THE URUGUAYAN CANINE BREED CIMARRON (Canis familiaris) USING RAPD MARKERS / ANÁLISIS DE DOS POBLACIONES DE CANINOS (Canis familiaris) DE LA RAZA CIMARRÓN URUGUAYO UTILIZANDO RAPDs COMO MARCADORES MOLECULARES
Llambí D, Silvia; Gagliardi B, Rosa; Martínez T, Mónica; Estevez C, Jorge; Gorozurreta O, Ana; Costa De F, Gabriela; Bianco Y, Carolina; Artigas S, Rody; Arruga L, María Victoria.
  • Llambí D, Silvia; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Gagliardi B, Rosa; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Martínez T, Mónica; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Estevez C, Jorge; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Gorozurreta O, Ana; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Costa De F, Gabriela; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Bianco Y, Carolina; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Artigas S, Rody; University of Uruguay. Faculty of Veterinary. Laboratory of Genetics. Montevideo. UY
  • Arruga L, María Victoria; University of Zaragoza. Faculty of Veterinary. Laboratory of Cytogenetics and Molecular Genetics. Zaragoza. ES
Rev. MVZ Córdoba ; 13(3): 1464-1468, sep.-dic. 2008. ilus, graf, tab
Article Dans Anglais | LILACS, COLNAL | ID: lil-637120
ABSTRACT
Objective. To analyze the genetic variability in two populations of the native canine breed "Uruguayan Cimarron" from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei's average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.
RESUMEN
Objetivo. Analizar la variabilidad genética en dos poblaciones de caninos de la raza autóctona "Cimarrón uruguayo" provenientes de distintas zonas del País. Materiales y métodos. Se aisló ADN de 40 animales de la raza Cimarrón. Mediante la técnica de PCR se analizaron 11 marcadores moleculares RAPD (amplificación al azar de ADN polimórfico). Resultados. Se detectó un 14.9% de loci polimórficos. Se obtuvo un índice de bandas compartidas de 0.91, una distancia genética de 0.16 e índice promedio de diversidad de Nei's de 0.08. Conclusiones. Ambas poblaciones presentan una alta homogeneidad e identidad genética para estos marcadores y pertenecerían a un núcleo genético común.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Chiens Pays comme sujet: Amérique du Sud / Uruguay langue: Anglais Texte intégral: Rev. MVZ Córdoba Thème du journal: Médecine vétérinaire Année: 2008 Type: Article Pays d'affiliation: Espagne / Uruguay Institution/Pays d'affiliation: University of Uruguay/UY / University of Zaragoza/ES

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Chiens Pays comme sujet: Amérique du Sud / Uruguay langue: Anglais Texte intégral: Rev. MVZ Córdoba Thème du journal: Médecine vétérinaire Année: 2008 Type: Article Pays d'affiliation: Espagne / Uruguay Institution/Pays d'affiliation: University of Uruguay/UY / University of Zaragoza/ES