Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L. )
Electron. j. biotechnol
;
14(5): 5-5, Sept. 2011. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-640512
ABSTRACT
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4 percent) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6 percent) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Arachis
/
Réaction de polymérisation en chaîne
/
Basse température
/
Hybridation d'acides nucléiques
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Electron. j. biotechnol
Thème du journal:
Biotechnologie
Année:
2011
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Jilin Academy of Agricultural Sciences/CN
/
Ocean University of China/CN
/
Shandong Peanut Research Institute/CN
/
Zhengzhou Tobacco Research Institute of CNTC/CN
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS