An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1
Mem. Inst. Oswaldo Cruz
;
107(5): 571-577, Aug. 2012. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-643740
ABSTRACT
Although several studies have evaluated the role of p16INK4a as a diagnostic marker of cervical intraepithelial neoplasia (CIN) and its association with disease progression, studies regarding the role of p16INK4a in human immunodeficiency virus (HIV)-infected patients remain scarce. The present study was designed to determine the potential utility of p16INK4a as a diagnostic marker for CIN and invasive cervical cancer in HIV-positive and negative cervical specimens. An immunohistochemical analysis of p16INK4a was performed in 326 cervical tissue microarray specimens. Performance indicators were calculated and compared using receiving operating characteristics curve (ROC)/area under the curve. In HIV-1-negative women, the percentage of cells that was positive for p16INK4a expression was significantly correlated with the severity of CIN (p < 0.0001). A ROC curve with a cut-off value of 55.28% resulted in a sensitivity of 89%, a specificity of 81%, a positive predictive value of 91% and a negative predictive value of 78%. HIV-seropositive women exhibited decreased expression of p16INK4a in CIN2-3 specimens compared with HIV-negative specimens (p = 0.031). The ROC data underscore the potential utility of p16INK4a under defined conditions as a diagnostic marker for CIN 2-3 staging and invasive cervical cancer. HIV-1 infection, however, is associated with relatively reduced p16INK4a expression in CIN 2-3.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Marqueurs biologiques tumoraux
/
Dysplasie du col utérin
/
Tumeurs du col de l'utérus
Type d'étude:
Etude diagnostique
/
Étude observationnelle
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Adulte
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2012
Type:
Article
Pays d'affiliation:
Brésil
/
États-Unis d'Amérique
Institution/Pays d'affiliation:
Fonte Medicina Diagnóstica/BR
/
Instituto Fernandes Figueira-Fiocruz/BR
/
Johns Hopkins Bloomberg School of Public Health/US
/
Ohio State University/US
/
Universidade Federal do Rio de Janeiro/BR
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