Characterizations of a new Cordyceps cicadae isolate and production of adenosine and cordycepin
Braz. j. microbiol
;
43(2): 449-455, Apr.-June 2012. ilus
Article
Dans Anglais
| LILACS
| ID: lil-644458
ABSTRACT
Cordyceps is a fastidious pathogenic fungus infecting insects, and recent years have witnessed rapid progress in its medical properties. In this study, a wild isolate, C. cicadae MP12, was characterized through in vitro cultivation and its nuclear small-subunit (SSU) ribosomal DNA (rDNA) data. In vitro culture of C. cicadae MP12 was established by growing its fruiting bodies in a solid matrix. C. cicadae MP12 was inoculated into Cryptotympana atrata cicada pupae for in vivo culture, where the fungi developed its fruiting body as well. The contents of adenosine and cordycepin in dried fruiting bodies after culture were 1421.45µg/g and 1398.12 µg/g, respectively. Therefore, the established cultures from this study could be used for the production of various medically important metabolic substances.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
ADN ribosomique
/
Adénosine
/
Réaction de polymérisation en chaîne
/
Cordyceps
/
Champignons
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2012
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
China Agricultural University/CN
/
Zhejiang Agricultural and Forestry University/CN
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