Hydrolysis of various thai agricultural biomasses using the crude enzyme from Aspergillus aculeatus iizuka FR60 isolated from soil
Braz. j. microbiol
;
43(2): 456-466, Apr.-June 2012. ilus, graf, tab
Article
Dans Anglais
| LILACS
| ID: lil-644459
ABSTRACT
In this study, forty-two fungi from soil were isolated and tested for their carboxymethyl cellulase (CMCase) and xylanase activities. From all isolates, the fungal isolate FR60, which was identified as Aspergillus aculeatus Iizuka, showed high activities in both CMCase and xylanase with 517 mU/mg protein and 550 mU/mg protein, respectively. The crude enzyme from A. aculeatus Iizuka FR60 could hydrolyze several agricultural residues such as corncob, and sweet sorghum leaf and stalk at comparable rates with respect to the tested commercial enzymes and with a maximum rate in rice hull hydrolysis (29 μg sugar g-1 dry weight substrate mg-1 enzyme hr-1). The highest amount of glucose was obtained from corncob by using the crude enzyme from A. aculeatus Iizuka FR60 (10.1 g/100 g dry substrate). From overall enzymatic treatment results, the lowest sugar yield was from rice hulls treatment (1.6 g/100 g dry weight) and the highest amount of reducing sugar was obtained from rice straw treatment (15.3 g/100 g dry weight). Among tested agricultural wastes, rice hull could not be effectively hydrolyzed by enzymes, whereas sugarcane leaf and stalk, and peanut shell could be effectively hydrolyzed (30-31% total sugar comparing with total sugar yield from acid treatment).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Aspergillus
/
Oryza
/
Oxycellulose
/
Zea mays
/
Aliments
Type d'étude:
Études d'évaluation
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2012
Type:
Article
Pays d'affiliation:
Thaïlande
Institution/Pays d'affiliation:
Khon Kaen University/TH
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS