A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Qiu, Shanlian; Chen, Jichen; Lin, Si; Lin, Xinjian

Qiu, Shanlian; Chen, Jichen; Lin, Si; Lin, Xinjian.

Qiu, Shanlian; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN
Chen, Jichen; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN
Lin, Si; Fuzhou University. College of biological science and technology. Fuzhou. CN
Lin, Xinjian; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN

Braz. j. microbiol ; 43(2): 649-652, Apr.-June 2012. ilus, tab

Article Dans Anglais | LILACS | ID: lil-644482

ABSTRACT

ABSTRACT

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

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Backed gel; DNA; Silver staining

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Polyesters / Argent / Coloration et marquage / Techniques in vitro / ADN / Alcalanité du Sol / Agents colorants / Gels Type d'étude: Guide de pratique langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2012 Type: Article / descriptif de projet Pays d'affiliation: Chine Institution/Pays d'affiliation: Fuzhou University/CN / Soil and Fertilizer Institute/CN

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