Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
Electron. j. biotechnol
;
15(4): 8-8, July 2012. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-646958
ABSTRACT
Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC +/- 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Phénols
/
Poaceae
langue:
Anglais
Texte intégral:
Electron. j. biotechnol
Thème du journal:
Biotechnologie
Année:
2012
Type:
Article
Pays d'affiliation:
Chili
/
États-Unis d'Amérique
Institution/Pays d'affiliation:
Instituto de Investigaciones Agropecuarias/CL
/
Universidad Técnica Federico Santa María-Pontificia Universidad Católica de Valparaíso/CL
/
Universidad de Santiago de Chile/CL
/
University of California-Davis/US
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