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Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
Moretti, Marcia M. S; Bocchini-Martins, Daniela A; Silva, Roberto Da; Rodrigues, André; Sette, Lara D; Gomes, Eleni.
  • Moretti, Marcia M. S; Universidade Estadual Paulista. Laboratório de Bioquímica e Microbiologia Aplicada. São José do Rio Preto. BR
  • Bocchini-Martins, Daniela A; Universidade Estadual Paulista. Departamento de Bioquímica. Araraquara. BR
  • Silva, Roberto Da; Universidade Estadual Paulista. Laboratório de Bioquímica e Microbiologia Aplicada. São José do Rio Preto. BR
  • Rodrigues, André; Universidade Estadual Paulista. Departamento de Bioquímica e Microbiologia. Rio Claro. BR
  • Sette, Lara D; Universidade Estadual Paulista. Departamento de Bioquímica e Microbiologia. Rio Claro. BR
  • Gomes, Eleni; Universidade Estadual Paulista. Laboratório de Bioquímica e Microbiologia Aplicada. São José do Rio Preto. BR
Braz. j. microbiol ; 43(3): 1062-1071, July-Sept. 2012. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-656675
ABSTRACT
Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40ºC to 65ºC and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Xylanes / Carbone / Conditions du Sol / Cellulases / Deuteromycota / Fermentation / Fongicides industriels Type d'étude: Études d'évaluation langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2012 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual Paulista/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Xylanes / Carbone / Conditions du Sol / Cellulases / Deuteromycota / Fermentation / Fongicides industriels Type d'étude: Études d'évaluation langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2012 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual Paulista/BR