Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis
Mem. Inst. Oswaldo Cruz
; 107(8): 1070-1072, Dec. 2012. ilus
Article
de En
| LILACS
| ID: lil-660659
Bibliothèque responsable:
BR1.1
ABSTRACT
Trypanosomatidae is a family of early branching eukaryotes harbouring a distinctive repertoire of gene expression strategies. Functional mature messenger RNA is generated via the trans-splicing and polyadenylation processing of constitutively transcribed polycistronic units. Recently, trans-splicing of pre-small subunit ribosomal RNA in the 5' external transcribed spacer region and of precursor tRNAsec have been described. Here, we used a previously validated semi-nested reverse transcription-polymerase chain reaction strategy to investigate internal transcribed spacer (ITS) I acceptor sites in total RNA from Leishmania (Leishmania) amazonensis. Two distinct spliced leader-containing RNAs were detected indicating that trans-splicing reactions occur at two AG acceptor sites mapped in this ITS region. These data provide further evidence of the wide spectrum of RNA molecules that act as trans-splicing acceptors in trypanosomatids.
Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Leishmania mexicana
/
Précurseurs des ARN
/
ARN des protozoaires
/
Espaceur de l'ADN ribosomique
/
Sites d'épissage d'ARN
Type d'étude:
Diagnostic_studies
langue:
En
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
MEDICINA TROPICAL
/
PARASITOLOGIA
Année:
2012
Type:
Article