Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
Rev. Soc. Bras. Med. Trop
;
46(1): 34-38, Jan.-Feb. 2013. graf, tab
Article
Dans Anglais
| LILACS
| ID: lil-666791
ABSTRACT
INTRODUCTION:
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil.METHODS:
This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST).RESULTS:
From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE.CONCLUSIONS:
The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Infections à staphylocoques
/
Infections des tissus mous
/
Staphylococcus aureus résistant à la méticilline
Type d'étude:
Étude observationnelle
/
Étude de prévalence
/
Facteurs de risque
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Rev. Soc. Bras. Med. Trop
Thème du journal:
Médecine tropicale
Année:
2013
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Ciências da Saúde de Porto Alegre/BR
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