A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
Mem. Inst. Oswaldo Cruz
;
108(3): 390-391, maio 2013.
Article
Dans Anglais
| LILACS
| ID: lil-676966
ABSTRACT
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Réaction de polymérisation en chaîne
/
Stenotrophomonas maltophilia
Type d'étude:
Guide de pratique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2013
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Pontifícia Universidade Católica do Rio Grande do Su/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS