Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
Mem. Inst. Oswaldo Cruz
;
108(4): 512-515, jun. 2013. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-678280
ABSTRACT
The aim of this study was to determine the genetic diversity of Giardia duodenalis present in a human population living in a northern Ecuadorian rain forest. All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were subsequently genotyped using NlaIV and RsaI enzymes. The gdh gene was successfully amplified in 74 of 154 ELISA positive samples; 69 of the 74 samples were subsequently genotyped. Of these 69 samples, 42 (61%) were classified as assemblage B (26 as BIII and 16 as BIV), 22 (32%) as assemblage A (3 as AI and 19 as AII) and five (7%) as mixed AII and BIII types. In this study site we observe similar diversity in genotypes to other regions in Latin America, though in contrast to some previous studies, we found similar levels of diarrheal symptoms in those individuals infected with assemblage B compared with those infected with assemblage A.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Giardiase
/
Giardia
/
Glutamate dehydrogenase
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Equateur
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2013
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Équateur
Institution/Pays d'affiliation:
Universidad Central del Ecuador/EC
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