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Somatic embryogenesis of Neoglaziovia variegata (Arruda) Mez, an important source of fiber from native Brazilian bromeliads
Silveira, Daniela Garcia; Lino, Lucimeire Souza Morais; Souza, Antônio da Silva; Souza, Fernanda Vidigal Duarte.
  • Silveira, Daniela Garcia; Instituto Federal Baiano. Guanambi. BR
  • Lino, Lucimeire Souza Morais; Instituto Federal Baiano. Guanambi. BR
  • Souza, Antônio da Silva; Instituto Federal Baiano. Guanambi. BR
  • Souza, Fernanda Vidigal Duarte; Instituto Federal Baiano. Guanambi. BR
Braz. arch. biol. technol ; 56(4): 547-555, July-Aug. 2013. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-684506
ABSTRACT
Neoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from the different induction media were transferred to MS medium supplemented with 30 g.L-1 sucrose, 2.4 g.L-1 Phytagel®, 0.53 µM α-naphthalene acetic acid (NAA) and 0.88 µM of 6-benzylaminopurine (BAP). The regenerated plants presented normal growth; however, many embryos did not fully develop.


Texte intégral: Disponible Indice: LILAS (Amériques) Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. arch. biol. technol Thème du journal: Biologie Année: 2013 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Instituto Federal Baiano/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. arch. biol. technol Thème du journal: Biologie Année: 2013 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Instituto Federal Baiano/BR