Identification and characterisation of microRNAs in young adults of Angiostrongylus cantonensis via a deep-sequencing approach
Mem. Inst. Oswaldo Cruz
; 108(6): 699-706, set. 2013. tab, graf
Article
Dans En
| LILACS
| ID: lil-685489
Responsable en Bibliothèque :
BR1.1
ABSTRACT
Angiostrongylus cantonensis is an important causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. MicroRNAs (miRNAs) are small non-coding RNAs that participate in a wide range of biological processes. This study employed a deep-sequencing approach to study miRNAs from young adults of A. cantonensis. Based on 16,880,456 high-quality reads, 252 conserved mature miRNAs including 10 antisense miRNAs that belonging to 90 families, together with 10 antisense miRNAs were identified and characterised. Among these sequences, 53 miRNAs from 25 families displayed 50 or more reads. The conserved miRNA families were divided into four groups according to their phylogenetic distribution and a total of nine families without any members showing homology to other nematodes or adult worms were identified. Stem-loop real-time polymerase chain reaction analysis of aca-miR-1-1 and aca-miR-71-1 demonstrated that their level of expression increased dramatically from infective larvae to young adults and then decreased in adult worms, with the male worms exhibiting significantly higher levels of expression than female worms. These findings provide information related to the regulation of gene expression during the growth, development and pathogenesis of young adults of A. cantonensis.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Infections à Strongylida
/
Analyse de séquence d'ARN
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Angiostrongylus cantonensis
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MicroARN
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Séquençage nucléotidique à haut débit
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
MEDICINA TROPICAL
/
PARASITOLOGIA
Année:
2013
Type:
Article
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Project document