Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients
Clinics
;
68(9): 1206-1209, set. 2013. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-687769
ABSTRACT
OBJECTIVE:
This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.METHODS:
A total of 475 respiratory samples, 236 from patients in a hematopoietic stem cell transplantation program and 239 from kidney transplant patients, were analyzed by a direct fluorescence assay and the Centers for Disease Control real-time polymerase chain reaction protocol for influenza A and B detection.RESULTS:
Influenza detection using either method was 7.6% in the hematopoietic stem cell transplant group and 30.5% in the kidney transplant patient group. Influenza detection by real-time polymerase chain reaction yielded a higher positive rate compared with fluorescence than that reported by other studies, and this difference was more pronounced for influenza A. The fluorescence assay sensitivity, specificity, positive and negative predictive values, and kappa coefficient were 17.6%, 100%, 1, 0.83, and 0.256, respectively, and lower detection rates occurred in the kidney transplant patients.CONCLUSIONS:
The real-time polymerase chain reaction performance and the associated turnaround time for a large number of samples support the choice of this method for use in different routine diagnostic settings and influenza surveillance in high-risk patients. .
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Virus de la grippe A
/
Virus influenza B
/
Sujet immunodéprimé
/
Technique d'immunofluorescence directe
/
Grippe humaine
/
Réaction de polymérisation en chaine en temps réel
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Études d'évaluation
/
Guide de pratique
/
Étude observationnelle
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Adulte
/
Humains
langue:
Anglais
Texte intégral:
Clinics
Thème du journal:
Médicament
Année:
2013
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Federal University of Sao Paulo/BR
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