A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth

ABSTRACT

Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonicsolution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysisemploying 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to thevarious media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametricassay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity(neutral red test), and total cell density (crystal violet dye exclusion test). Results from each testwere compared by one-way analysis of variance (ANOVA). Results: Statistical analysis showedthat whole milk and HBSS were the most effective media in maintaining cell viability at all testedtimes (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects. Conclusions: Thisstudy shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3fibroblasts as demonstrated by three different cell viability tests.