Poly (2'-0-methyladenylic acid) as template of reverse transcriptase
Arq. biol. tecnol
;
31(2): 313-20, 1988. tab
Article
Dans Anglais
| LILACS
| ID: lil-69596
RESUMO
Poly (2'-O-methyladenylic acid), poly (Am), is recognized as template in the reaction catalyzed by avian myeloblastosis virus, AMV, reverse transcriptase better in the presence of Mn than Mg as divalent cation. An apparent KM value of 2.5 x 10 M in relation to TTP and an inhibition of the reaction at poly (Am concentration higher than 5 microng/ml have been observed. Ethidium bromide, EtBr, tetramethyl ethidium bromide, TMEtBr, dideoxy TTP, and novobiocin are inhibitors of the poly (Am)-directed reverse transcriptase reaction in the presence of Mn
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Indice:
LILAS (Amériques)
Sujet Principal:
Poly A
/
Matrices (génétique)
/
RNA-directed DNA polymerase
langue:
Anglais
Texte intégral:
Arq. biol. tecnol
Thème du journal:
Biologie
/
Biotechnologie
Année:
1988
Type:
Article
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