Genetic similarity between APEC and Escherichia coli strains isolated from Guaruba guarouba in a survey on healthy captive psittacine birds / Similaridade genética entre APEC e cepas de Escherichia coli isoladas de Guaruba guarouba em um estudo com psitacídeos hígidos de cativeiro
Braz. j. vet. res. anim. sci
;
50(2): 145-151, 2013.
Article
Dans Anglais
| LILACS
| ID: lil-696347
ABSTRACT
The role of psittacine birds as a reservoir of avian pathogenic Escherichia coli (APEC) is not known but would be helpful in understanding the human animal interface, since the enteric microbiota of these birds consists of Gram positive bacteria. The aim of this study was to identify the presence of APEC in feces of clinically healthy Guaruba guarouba. To do this, we isolated and analyzed E. coli from cloacal fecal samples taken from 87 psittacine birds from six zoologic parks, three commercial breeders and one conservation breeder. Of the 87 birds examined, 46 (52.87%) presented E. coli in feces. The presence of the following eight virulence genes was determined by the polymerase chain reaction (PCR) irp2, iucD, iss, vat, cvi/cva, tsh, astA, and papC, and 29 (63.04%) of 46 E. coli isolates tested were positive at least one of the eight genes studied. The frequency of virulence genes observed in isolates of E. coli were 32.6% (15/46) irp2, 26% (12/46) iucD, 19.5% iss (9/46), 17.4% vat (8/46), 17.4% cvi/cva (8/46), 8.7% tsh (4/46), 4.4% astA (2/46) and 0% papC (0/46). The isolates were grouped in 13 genotypic profiles according to virulence gene combinations, but only 2 isolates were classified as APEC, with the pattern iuc, iss, cvi/cva, irp + and iuc, iss, cvi/cva, irp, tsh, vat +. This study reveals the presence of APEC in clinically healthy captive G. guarouba, suggesting that these psittacine birds may act as reservoir for pathogenic microorganisms. Epidemiological studies are needed to determine the relevance of this species as a reservoir and the implications for conservation of endangered species G. guarouba.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Virulence
/
Épidémiologie
/
Génétique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. vet. res. anim. sci
Thème du journal:
Médecine vétérinaire
Année:
2013
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Governo do Estado de São Paulo/BR
/
Parque Zoológico Municipal Quinzinho de Barro/BR
/
Prefeitura de São Paulo/BR
/
Universidade de São Paulo/BR
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