Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the malachite green decolourisation assay
Mem. Inst. Oswaldo Cruz
;
108(8): 1021-1023, 6/dez. 2013. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-697148
ABSTRACT
Early detection of drug resistance in Mycobacterium tuberculosis isolates allows for earlier and more effective treatment of patients. The aim of this study was to investigate the performance of the malachite green decolourisation assay (MGDA) in detecting isoniazid (INH) and rifampicin (RIF) resistance in M. tuberculosis clinical isolates. Fifty M. tuberculosis isolates, including 19 multidrug-resistant, eight INH-resistant and 23 INH and RIF-susceptible samples, were tested. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and agreement of the assay for INH were 92.5%, 91.3%, 92.5%, 91.3% and 92%, respectively. Similarly, the sensitivity, specificity, PPV, NPV and agreement of the assay for RIF were 94.7%, 100%, 100%, 96.8% and 98%, respectively. There was a major discrepancy in the tests of two isolates, as they were sensitive to INH by the MGDA test, but resistant by the reference method. There was a minor discrepancy in the tests of two additional isolates, as they were sensitive to INH by the reference method, but resistant by the MGDA test. The drug susceptibility test results were obtained within eight-nine days. In conclusion, the MGDA test is a reliable and accurate method for the rapid detection of INH and RIF resistance compared with the reference method and the MGDA test additionally requires less time to obtain results.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Rifampicine
/
Magenta I
/
Multirésistance bactérienne aux médicaments
/
Isoniazide
/
Mycobacterium tuberculosis
/
Antituberculeux
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Étude de dépistage
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2013
Type:
Article
Pays d'affiliation:
Turquie
Institution/Pays d'affiliation:
Ondokuz Mayis University/TR
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