COI barcode versus morphological identification of Culex ( Culex ) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil
Mem. Inst. Oswaldo Cruz
;
108(supl.1): 110-122, 2013. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-697832
ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Classification
/
Complexe IV de la chaîne respiratoire
/
Culex
/
Codage à barres de l'ADN pour la taxonomie
/
Systèmes d'identification animale
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Argentine
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2013
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Argentine
Institution/Pays d'affiliation:
Universidad Nacional de Cordoba/AR
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