Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
Braz. j. microbiol
; 44(3): 823-828, July-Sept. 2013. ilus
Article
Dans En
| LILACS
| ID: lil-699794
Responsable en Bibliothèque :
BR1.1
ABSTRACT
In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Électrophorèse en champ pulsé
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Technique RAPD
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Typage moléculaire
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Klebsiella pneumoniae
Type d'étude:
Clinical_trials
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Diagnostic_studies
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Evaluation_studies
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Guideline
Limites du sujet:
Female
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Humans
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Male
langue:
En
Texte intégral:
Braz. j. microbiol
Thème du journal:
MICROBIOLOGIA
Année:
2013
Type:
Article