Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee
Braz. j. microbiol
;
44(3): 717-722, July-Sept. 2013. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-699804
ABSTRACT
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Abeilles
/
Lactobacillus
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2013
Type:
Article
Pays d'affiliation:
Malaisie
Institution/Pays d'affiliation:
University Putra Malaysia/MY
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