Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
Acta cir. bras
;
29(3): 186-192, 03/2014. graf
Article
Dans Anglais
| LILACS
| ID: lil-703525
ABSTRACT
To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS:
Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm 2.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Stress oxydatif
/
Ischémie
/
Poumon
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2014
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Centro Universitário São Camilo/BR
/
Universidade Federal da Grande Dourados/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de Mato Grosso do Sul/BR
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